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| Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003 |
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| Nóbrega Jr.,J.E.; Rossetto,R.; Matos,M.H.T.; Chaves,R.N.; Magalhães,D.M.; Lima-Verde,I.B.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.; Gonçalves,P.B.D.; Oliveira,J.F.C.. |
| This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P<0.05) in the percentage of primordial follicles in all groups... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preservation; Preantral follicles; Sphingosine 1-phosphate; Follicular activation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000400977 |
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